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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
26/01/2024 |
Actualizado : |
26/01/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
Complemento del título : |
Original full article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
ISSN : |
1983-0246 |
DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500 001 1064440 005 2024-01-26 008 2023 bl uuuu u00u1 u #d 022 $a1983-0246 024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI 100 1 $aPICCOLI, R.J. 245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- 520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. 653 $aDiagnosis 653 $aImmunohistochemistry 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aPsittacosis 653 $aZoonosis 700 1 $aGRUCHOUSKEI, L. 700 1 $aBENINCA, A.L.V. 700 1 $aMAURENTE BERÓN, M. 700 1 $aCHENG, A.C. 700 1 $aDE ANDRADE, J.A. 700 1 $aFACCIN, M. 700 1 $aGRZEGOZEVSKI, A.P. 700 1 $aDA SILVA, G.C.R. 700 1 $aFRANÇA, J.C. 700 1 $aFERNANDES, N.L.M. 700 1 $aDE CARVALHO, A.L. 700 1 $aVIOTT, A.M. 773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
13/11/2017 |
Actualizado : |
24/05/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
MAESO, D.; FERNÁNDEZ, A.; WALASEK, W. |
Afiliación : |
DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALFREDO JOSÉ FERNÁNDEZ TECHERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILMA GLADYS WALASEK UVIEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Control de la mancha bacteriana del tomate (Xanthomonas spp.) en cultivo a campo para industria mediante aplicaciones foliares. 2015-2016. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Manejo sanitario en tomate y morrón bajo cubierta. Resultados experimentales 2015-2017. Jornada de divulgación. Las Brujas, Canelones (UY): INIA, 2017. pp. 33-42.. |
Serie : |
(Serie Actividades de Difusión; 778) |
ISSN : |
1688-9266 |
Idioma : |
Español |
Contenido : |
La mancha bacteriana del tomate (Xanthomonas spp.) es una de las enfermedades foliares más importantes del cultivo al aire libre. Hasta el momento el control se basa en la reiterada
aplicación foliar preventiva de productos, principalmente a base de cobre muchas veces mezclado con mancozeb. Desde hace algunos años INIA ha venido realizando trabajos experimentales tendientes a evaluar la eficiencia de productos alternativos aplicados a follaje para el control de esta enfermedad. |
Palabras claves : |
MANCHA BACTERIANA. |
Thesagro : |
ENFERMEDADES FOLIARES; TOMATE; XANTHOMONAS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7935/1/SAD-778-2017.-p.33-42.pdf
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Marc : |
LEADER 01374naa a2200217 a 4500 001 1057765 005 2018-05-24 008 2017 bl uuuu u00u1 u #d 022 $a1688-9266 100 1 $aMAESO, D. 245 $aControl de la mancha bacteriana del tomate (Xanthomonas spp.) en cultivo a campo para industria mediante aplicaciones foliares. 2015-2016. 260 $c2017 490 $a(Serie Actividades de Difusión; 778) 520 $aLa mancha bacteriana del tomate (Xanthomonas spp.) es una de las enfermedades foliares más importantes del cultivo al aire libre. Hasta el momento el control se basa en la reiterada aplicación foliar preventiva de productos, principalmente a base de cobre muchas veces mezclado con mancozeb. Desde hace algunos años INIA ha venido realizando trabajos experimentales tendientes a evaluar la eficiencia de productos alternativos aplicados a follaje para el control de esta enfermedad. 650 $aENFERMEDADES FOLIARES 650 $aTOMATE 650 $aXANTHOMONAS 653 $aMANCHA BACTERIANA 700 1 $aFERNÁNDEZ, A. 700 1 $aWALASEK, W. 773 $tIn: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Manejo sanitario en tomate y morrón bajo cubierta. Resultados experimentales 2015-2017. Jornada de divulgación. Las Brujas, Canelones (UY): INIA, 2017. pp. 33-42..
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